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. 2014 Dec 23;4(1):e215. doi: 10.1038/mtna.2014.66

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Copyright © 2014 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Strategy for rolling circle transcription (RCT) site-specific disconnection (SSD) synthesis. cDNA is circularized to form a circular DNA template, and long RNA strands are generated by RCT consisting of tandem repeats of desired RNA. With the help of Aid-DNA, RNase H disconnects the transcript to generate thousands of copies of the desired RNA.