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. 2014 Dec 23;4(1):e215. doi: 10.1038/mtna.2014.66

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Copyright © 2014 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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MicroRNA synthesized by rolling circle transcription (RCT) site-specific disconnection (SSD) with various concentrations of circular cDNA template. (a) Time course of RCT in the absence of inorganic pyrophosphatase (IPP). (b) Effect of IPP on the amount of RNA products. Lane L, 25 nt RNA marker; Lane S, standard sample of chemically synthesized mir-16 (1 μl, 100 μmol/l). In (b), RCT was processed for 4 hours, with 0.1 μmol/l Aid-DNA-16-1 and 1.0 U/ml IPP.