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. 2014 Dec 16;3(12):e216. doi: 10.1038/mtna.2014.68

Figure 4.

Figure 4

Nucleotide sequence analysis of cloned cccDNA. (a) The target sequence (plus strand) of sg5 guide RNA on the HBV genome and the position of five selected mutations identified in cloned PCR fragments derived from cccDNA of HBV-infected cells expressing sg5 are inidcated. Deletions are indicated with dots, insertions with a plus sign. Position 2,987 corresponds to the 3′ end of sgRNA 5 (sg5) abutting the PAM sequence (GTT). For the positions of the pol and X genes on the HBV genome, see Figure 1b.