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. 2014 Dec 16;3(12):e217. doi: 10.1038/mtna.2014.70

Figure 3.

Figure 3

eIF4e aptamers inhibit translation. (a) Insect cell transcription and translation kit using a luciferase template and stated dilution of aptamer or (b) 20 µmol/l of aptamer 1 in the presence of 120 µmol/l BSA or human eIF4e. NS is a random oligonucleotide that can form a hairpin structure. (c) Number of reads from eIF4e Aptamer 1 cluster round 8 that deviated at specific positions from the most abundant member of the cluster. Red letters indicate highly conserved nucleotide, green indicates a position that has relatively more substitutions. (d) MFold prediction of the secondary structure of eIF4e Apt 1 with the associated bases highlighted in red or green. (e) Insect cell transcription and translation assay with eIF4e Apt 2 and eIF4e Apt 1 and mutants with a single modification at specific positions. All error bars reflect standard deviations. *P < 0.05; **P < 0.01; ***P < 0.001; two-tailed Student's t-test. For a, P values refer to comparisons against control/no aptamer.