Figure 5.

Ligation of aptamers enhances activity. (a) Aptamers phosphorylated with T4 DNA ligase and ligated at 4 µmol/l applied to the insect cell TnT kit and luciferase levels measured 4 hours after application. Aptamers were heated and cooled before ligation, two samples were heated to 85 °C after ligation and cooled again instead of directly being applied to the TnT mix. (b) HeLa cells were transfected with aptamers ligated or aptamers alone at 20 nmol/l with lipofectamine 2000 and measured 2 days after transfection with Cell Titer Blue.