FIG 3.

Transient heat shock leads to telomere shortening in wild-type cells. Strain SLY20 was subjected to heat shock at 39°C for 40 min and then was grown at 30°C for 6 days. Genomic DNA was isolated at different time intervals (as marked at the top) and subjected to XhoI digestion, and telomere length was measured using Southern blot hybridization. The experiment was repeated with three independent colonies; two telomere blots are represented. The difference between the lengths of telomeres grown at 30°C and the 2-h post-HS sample is represented by solid lines.