TABLE 1.
Primers used in this study
| Primer name | Sequence | Purpose |
|---|---|---|
| OSB 125 | 5′ ATC CTC GAG CTG CAA CTC CTC AAT GTG TC 3′ | Forward primer used to amplify 429-bp SIR2UAS |
| OSB 193 | 5′ ATC CTC GAG GTA TAT GCT TAT ATG CAT GCG 3′ | Forward primer used to amplify 370-bp SIR2UAS |
| OSB 194 | 5′ATC CTC GAG CCA AGC TAC ATC TAG CAC TC 3′ | Forward primer used to amplify 307-bp SIR2UAS |
| OSB 126 | 5′ ATC CTC GAG CTT TGG CCG CCA GTT GCG 3′ | Forward primer used to amplify 200-bp SIR2UAS |
| OSB 87 | 5′ ATC GGA TCC GGT CAT CCA GCT TTA ATG TGC CG 3′ | Common reverse primer used to amplify all above deletion constructs of SIR2UAS |
| OSB 203 | 5′ GAC GGA TCC ATG AAT TAT AAC TGC GAA ATA C 3′ | Forward primer used to amplify CUP9 for cloning in pESC-MYC-tagged vector |
| OSB 204 | 5′ CGA GTC GAC ATT CAT ATC AGG GTT GGA TAG 3′ | Reverse primer used to amplify CUP9 for cloning in pESC-MYC-tagged vector |
| OSB 164 | 5′ CTT TTA TGC TAA CAA CCT TCG AGA ATA GTT ACA TTC GAA GCG GAT CCC CGG GTT AAT TAA 3′ | Forward primer used for CUP9 knockout |
| OSB 165 | 5′ TAT AAT TAT ATG AAT ATT TAA GTA ATG CAT TGA TAA GTG AGA ATT CGA GCT CGT TTA AAC 3′ | Reverse primer used for CUP9 knockout |
| OSB 170 | 5′ AAG TTT CAT ACA TAA TTA ACA AAA TTC GTT TGT TGC GGG GCG GAT CCC CGG GTT AAT TAA 3′ | Forward primer used for SUM1 knockout |
| OSB 171 | 5′ TTT TAT CTA TTC TCG AAA CTG CCC CAA CGT ACG GAC CAG CGA ATT CGA GCT CGT TTA AAC 3′ | Reverse primer used for SUM1 knockout |
| OSB 173 | 5′ ACT GAA AAC GGT AAA GTA GGT TTG TTT AAA TTG ACT TAA GCG GAT CCC CGG GTT AAT TAA 3′ | Forward primer used for RIM101 knockout |
| OSB 174 | 5′ GCA AAG AAA CAA CTA AGA ATA AAA TAT CCG ACA ATC CAT AGA ATT CGA GCT CGT TTA AAC 3′ | Reverse primer used for RIM101 knockout |
| OSB 161 | 5′ CAA AAT CAT CCT TAT ATA ACC CTG GTA AGG TCC TTT TGT CCG GAT CCC CGG GTT AAT TAA 3′ | Forward primer used for SOK2 knockout |
| OSB 162 | 5′ GAT TAA AGT AAC ATA ATT ATC CAA GGA ATT CAT AGT TGT TGA ATT CGA GCT CGT TTA AAC 3′ | Reverse primer used for SOK2 knockout |
| OSB 196 | 5′ GCT GGA AGA ATT GAA AAA GCT ATC CAA CCC TGA TAT GAA TCG GAT CCC CGG GTT AAT TAA 3′ | Forward primer used to generate MYC tag at the portion of CUP9 corresponding to the C terminus at the chromosomal locus |
| OSB 197 | 5′ TAT AAT TAT ATG AAT ATT TAA GTA ATG CAT TGA TAA GTG AGA ATT CGA GCT CGT TTA AAC 3′ | Reverse primer used to generate MYC tag at the portion of CUP9 corresponding to the C terminus at the chromosomal locus |
| OSB 16 | 5′ TGA CCA AAC TAC TTA CAA CTC C 3′ | Forward primer used to amplify ACT1 for real-time RT-PCR |
| OSB 14 | 5′ TTA GAA ACA CTT GTG GTG AAC G 3′ | Reverse primer used to amplify ACT1 for real-time RT-PCR |
| OSB 131 | 5′ CTG ATT AAT CGT GAT CCC GTC 3′ | Forward primer used to amplify SIR2 for real-time RT-PCR |
| OSB 132 | 5′ CTT AGA GGG TTT TGG GAT GTT C 3′ | Reverse primer used to amplify SIR2 for real-time RT-PCR |
| OSB 121 | 5′ CAA CTG ATG GAA ACC AGC C 3′ | Forward primer used to amplify LACZ for real-time RT-PCR |
| OSB 122 | 5′ TTA CGC GAA ATA CGG GCA G 3′ | Reverse primer used to amplify LACZ for real-time RT-PCR |
| OSB 189 | 5′ CTA ATG ACA ACG CGA ATA ATA C 3′ | Forward primer used to amplify CUP9 for real-time RT-PCR |
| OSB 190 | 5′ CAA TTC ATA TCA GGG TTG GAT AG 3′ | Reverse primer used to amplify CUP9 for real-time RT-PCR |
| OSB 19 | 5′ ATC ACG AGT AAG GAT CAA AG 3′ | Forward primer used to amplify YFR057w for real-time RT-PCR |
| OSB 20 | 5′ TTA TGG CTT TGT TAC GCT TG 3′ | Reverse primer used to amplify YFR057w for real-time RT-PCR |
| OSB 62 | 5′ AAT CGG CGG ATG GGT TGG 3′ | Forward primer used to amplify HMLα for real-time RT-PCR |
| OSB 63 | 5′ TCA TTC TTT CTT CTT TGC CAG 3′ | Reverse primer used to amplify HMLα for real-time RT-PCR |