FIG 7.

Mapping functional domains on the Bem4p protein. (A) In vitro pulldowns with versions of Bem4p fused to MBP with HIS-Cdc42p. (B) A region of Bem4p from amino acid residues 300 to 400 interacts with HIS-Cdc42p. (C) Versions of MBP-Bem4p that include amino acid residues 1 to 100 interact with GST-Cdc24p, as determined by in vitro pulldown. Controls for the pulldowns can be found in Fig. 6. (D) Impact of internal deletions within the Bem4p protein on the stability of the protein and filamentous-growth pathway activity. (E) Diagram of the Bem4p protein with Cdc24p and Cdc42p sites shown. One region of the protein that affects the stability of the protein is also shown.