FIG 8.

Bem4p interacts with the MAPKKK Ste11p. (A) (Left) Bem4p interacts with Ste11p by two-hybrid analysis but not Ras2p or Pbs2p. (Right) β-Galactosidase activity (in Miller units) of the two-hybrid reporter GAL7-lacZ was measured for the constructs shown. Values are averages from two experiments; the standard deviation was less than 10% between trials. (B) MBP-Bem4p interacts with GST-Ste11p in vitro. Fifty percent of the WCE (input) was used for the pull down. (C) MAPK activity (based on the β-galactosidase activity of the ste4 FUS1-lacZ reporter) in wild-type cells and the bem4Δ mutant containing the indicated versions of Ste50p and Ste11p. Asterisks denote P values of <0.05; the double asterisk denotes a P value of <0.01.