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. Author manuscript; available in PMC: 2015 Dec 18.
Published in final edited form as: Cell. 2014 Dec 18;159(7):1563–1577. doi: 10.1016/j.cell.2014.11.037

Figure 5. Activation of the cGAS/STING pathway of IFN induction.

Figure 5

(A) Western blot analysis of the phosphorylation of TBK1 and IRF-3 in BaxBak WT and KO cells treated with combined Bcl-2/caspase inhibitors (ABT-737 + Q-VD-OPH, 10 μM each), or transfected with HT-DNA as a positive control (3 μg/ml, 3h). Result representative of 3 independent experiments.

(B and C) Expression of IFNβ mRNA by IRF-3/7 double KO (B), MAVS KO (C) and control WT primary MEFs after 6h of treatment with vehicle (DMSO) or with ABT-737 + Q-VD-OPH (mean ± s.d. of triplicates, representative of 2 experiments). *, p<0.05; ns, not significant; pairwise comparisons following two-way ANOVA.

(D) cGAMP measurement in cell extracts of WT primary MEFs stimulated for 4h with vehicle (DMSO) or ABT-737 + Q-VD-OPH. Result representative of 2 independent experiments.

(E and F) Expression of IFNβ mRNA by cGAS WT and KO bone marrow-derived macrophages (E) and by STING WT and KO primary MEFs (F) after 6h of treatment with vehicle or ABT-737 + Q-VD-OPH (mean ± s.d. of 3 or 2 replicates, respectively).

See also Figure S7.