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. Author manuscript; available in PMC: 2015 Dec 18.
Published in final edited form as: Cell. 2014 Dec 18;159(7):1563–1577. doi: 10.1016/j.cell.2014.11.037

Figure 6. cGAS/STING-dependent constitutive ISG expression in the absence of active caspases.

Figure 6

(A) Western blot analysis of the phosphorylation of TBK1 and IRF-3 in Casp9 WT and KO cells treated for 6h with vehicle (DMSO), with the Bcl-2 inhibitor ABT-737 (10 μM), or transfected with HT-DNA as a positive control (3 μg/ml, 3h). Result representative of 3 independent experiments.

(B) Western blot analysis of STING in Casp9 WT and KO cells treated for 16h with the indicated concentrations of chloroquine.

(C) Caspase-9 KO mice were crossed with IRF-3/7 DKO and the expression of ISGs in embryo heads was measured by RT-PCR. Results shown are mean ± s.d. of 3 embryos for each genotype.

(D and E) STING WT and KO primary MEFs (D) or cGAS WT and KO bone marrow-derived macrophages (E) were treated with vehicle (DMSO) or with the caspase inhibitor Q-VD-OPH (10 μM) and ISG expression was measured 48h later by RT-PCR (mean ± s.d. of triplicates, representative of 2 independent experiments).

(F) Caspase-9 KO mice were crossed with MAVS KO and the expression of ISGs in embryo heads was measured by RT-PCR. Results shown are mean ± s.d. of 2 embryos for each genotype.

*, p<0.05; ns, not significant; pairwise comparisons following two-way ANOVA.