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. Author manuscript; available in PMC: 2015 Dec 18.
Published in final edited form as: Immunity. 2014 Nov 25;41(6):1052–1063. doi: 10.1016/j.immuni.2014.11.009

Figure 4. IL-17RA signaling is required for the growth of aberrant crypt foci.

Figure 4

Cdx2Cre-ERT2ApcF/F mice were injected with tamoxifen for intestinal tumor induction. (A) Q-RT-PCR analysis of IL-17A mRNA in colon tissues following tamoxifen injection. N=8. (B) Immunostaining of MUC2 in colon cryosections 1 week after tamoxifen injection. Tamoxifen-injected Cdx2Cre-ERT2 negative mice were used as controls. Arrows indicate areas of MUC2 loss. Representative images of 3 slides of each specimen are shown. (C) Cdx2Cre-ERT2 ApcF/F mice heterozygous (+/−) or null (−/−) for Il17ra were sacrificed for colon tumor count 4 weeks after tamoxifen injection. n=5. (D) Cdx2Cre-ERT2 ApcF/F mice were given tamoxifen via i.p. injection. Starting one day after the last tamoxifen dose, mice received i.p. injections of 500 ug of isotype control or anti-IL-17A antibody on a weekly basis. Colon tumors were counted 4 weeks after last tamoxifen injection. N=7. (E, F) Immunostaining of colon cryosections from Cdx2Cre-ERT2 ApcF/F mice heterozygous (+/−) or null (−/−) for Il17ra 2 weeks after tamoxifen injection. (F) Quantification of Ki-67 (n=4) and phospho-NF-κB p65 (P-p65; n=7) positive transformed cells in HMF of tamoxifen-induced ACF lesions. Data shown in arbitrary units (AU) represent averages ± S.E.M. * p < 0.05. Scale bar = 100 μm. See also Figure S6.