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. 2014 Nov 30;14:126. doi: 10.1186/s12935-014-0126-4

Figure 5.

Figure 5

Curcumin modulated different PI3K/Akt pathway in MCF-7 and MDA-MB-231 cells. (A) Analysis of p-Akt (1:5000), Akt (1:1000) changes induced by indicated curcumin for 24 h or 40 μM curcumin for indicated time in MCF-7 and MDA-MB-231 cells was took by western blotting. (B) MCF-7 cells were treated with 1 μM concentration of wortmannin for 1 h prior to treatment with curcumin (40 μM) for 6 h. Expression of p-Akt (1:5000) and SKP2 (1:1000) in indicated condition was examined by western blotting. Also shown is a blot for GAPDH as a loading control. (C) Analysis of p-Foxo1 (1:1000) and p-Foxo3a (1:1000) changes induced by 40 μM curcumin for indicated time in MCF-7 and MDA-MB-231 cells was taken by western blotting. Also shown is blot for β-actin as a loading control. The representative data of at least two independent experiments are shown.