FIG 7.

Deposition of S-layer-associated proteins in the envelope of wild-type and lcpB3 and lcpD mutant Bacillus anthracis. Spores from wild-type B. anthracis Sterne (WT with empty vector) or lcpB3 or lcpD mutant strains containing empty vector or complementing plasmid were germinated in BHI broth, incubated for 8 h, fixed in formalin, and stained with polyclonal anti-BslR (A) or anti-BslU (B) rabbit antibodies. Antibody binding and bacterial architecture were revealed with DyLight594-conjugated anti-rabbit secondary antibody and BODIPY-vancomycin, respectively. Differential interference contrast (DIC) and fluorescence microscopy images were acquired. Data sets were merged to reveal the location of the proteins in relation to the cell septa (BODIPY-vancomycin). As controls, bslR and bslU mutants were also stained with their respective antisera. Scale bar = 10 μm.