FIG 1.

Western blot analysis of germination protein levels in the L fraction of dormant and germinating PS4150 spores. The L fraction from spores was isolated, equal amounts of protein were run on SDS-PAGE gels, and Western blots were probed with various antisera. Lanes: 1, dormant spores; 2, spores after 15 min of germination; 3, spores after 60 min of germination; 4, spores after 120 min of germination. MW, molecular weight markers (in thousands). The band running just below SpoVAD is likely a breakdown product of this protein, which was reported previously (6, 7).