FIG 5.

Western blot analysis of germination protein levels in spores of strains KGB73 (PS4150 gerD-gfp) (A) and KGB114 (PS4150 gerAA-mCherry) (B). The L fraction of dormant and germinating spores was isolated, and equal amounts of protein were run on SDS-PAGE gels and subjected to Western blot analysis with various antisera. Lanes: 1, dormant-spore L fraction; 2, L fraction at 15 min of germination; 3, L fraction at 60 min of germination; 4, L fraction at 120 min of germination. The migration positions of molecular mass markers (in kDa) are shown to the left of the panels. Proteins labeled with asterisks indicate a fluorescent fusion protein that ran at a higher molecular mass than the native protein.