FIG 1.

EMSAs displaying the specific interaction of HetR with the binding site in the trpE promoter. The HetR concentration was 25 μM (A) or 2.5 μM (B), and the DNA concentration was 2 μM (A) or 0.3 μM (B). The DNA lengths are 29 bp (A), 124 bp (B; hetP), 268 bp (B; lacZ), and 155 bp (B; trpE and trpEmut). Nucleotide sequence comparison of the 29-bp DNA fragments utilized in EMSAs in this study (C). Transversion mutations of selected nucleotides are underlined. DNA sequences are presented in the 5′-to-3′ direction.