TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| Anabaena sp. | ||
| PCC 7120 | Wild type | Institut Pasteur Culture Collection |
| UHM101 | ΔpatA | 49 |
| UHM103 | ΔhetR | 22 |
| UHM335 | ΔtrpE | This study |
| E. coli JA221 | ΔtrpE, tryptophan auxotroph | 45 |
| Plasmids | ||
| pAM504 | Shuttle vector for replication in E. coli and Anabaena; Kmr Nmr | 30 |
| pAM1956 | Shuttle vector pAM504 with promoterless gfp | 33 |
| pRL277 | Suicide vector; Spr Smr | 12 |
| pRR159 | pAM504 with PhetN-hetN-YFP | 27 |
| pSW7848 | Source of araC-ParaBAD | 32 |
| pET28b+hetR | Expression vector for purifying polyhistidine epitope-tagged HetR; Kmr | 35 |
| pPJAV123 | pAM504 with NdeI site removed | 50 |
| pPJAV149 | pRL277 used to make UHM335 | This study |
| pPJAV153 | Shuttle vector pAM504 for creating copper-inducible C-terminal YFP translational fusions | 50 |
| pPJAV297 | pAM504 with PpetE-trpE translationally fused to YFP | This study |
| pPJAV360 | pAM504 with araC-ParaBAD | This study |
| pPJAV370 | pAM504 with ParaBAD-trpE | This study |
| pPJAV381 | pAM1956 with PtrpE | This study |
| pPJAV382 | pAM1956 with PtrpE with mutated HetR binding site | This study |
| pAHB112 | pRL277 with PtrpE-trpE | This study |
| pAHB113 | pRL277 with PtrpE-trpE with mutated HetR binding site | This study |
a
Km, kanamycin; Nm, neomycin; Sp, spectinomycin; Sm, streptomycin.