FIG 2.

MSX and MSO inhibit growth of an mddA::cat⁺ strain. The growth of S. enterica mddA⁺ and mddA1::cat⁺ strains was examined in the presence of MSX (10 μM or 50 μM) or MSO (50 μM or 100 μM), as indicated. NCE minimal medium with glycerol (22 mM) or nutrient broth was used in these experiments. Growth curves were determined using a microplate reader (Bio-Tek Instruments) as described in Materials and Methods. The following strains were analyzed: JE10079 (mddA⁺), JE18333 (mddA1::cat⁺), JE18961 (mddA1::cat⁺/pMDD8 mddA⁺), and JE19029 (mddA1::cat⁺/pmDD11 mddA⁺ [encoding SeMddA^E82Q]). The error bars represent standard deviations.