FIG 3.

The effect of increasing MSX concentration on mddA⁺ and mddA::cat⁺ strains is detrimental to growth. The growth of S. enterica mddA⁺ and mddA1::cat⁺ strains was examined with increasing concentrations of MSX, as indicated. The minimal medium used was NCE with glycerol (22 mM), and the rich medium used was nutrient broth. Growth curves were determined using a microplate reader (Bio-Tek Instruments) as described in Materials and Methods. The following strains were analyzed: JE10079 (mddA⁺) and JE18333 (mddA1::cat⁺). The error bars represent standard deviations.