FIG 5.

Glutamine and methionine counteract the deleterious effects of MSX and MSO on growth in the absence of MddA. The growth of S. enterica mddA⁺ and mddA1::cat⁺ strains was examined in the presence of MSX (10 μM) and MSO (50 μM), with the addition of glutamine (200 μM, 500 μM, or 1,000 μM) or methionine (500 μM) to the medium. NCE minimal medium supplemented with glycerol (22 mM) was used in these experiments. Growth curves were determined using a microplate reader (Bio-Tek Instruments) as described in Materials and Methods. The following strains were analyzed: JE10079 (mddA⁺) and JE18333 (mddA1::cat⁺). The error bars represent standard deviations.