FIG 8.

An mddA⁺ strain exhibits biphasic growth in minimal medium containing glutamine and MSX. (A) Growth of the S. enterica mddA⁺ strain in NCE minimal medium supplemented with glycerol (22 mM) was examined in the presence of glutamine (200 μM) and increasing concentrations of MSX (5 to 200 μM), as indicated. The strain analyzed was JE10079 (mddA⁺). (B) Growth of the S. enterica mddA⁺/pMDD8 mddA⁺ strain was examined in the same medium in the presence of MSX (50 μM) and increasing concentrations of inducer [l-(+)-arabinose; 50 to 500 μM], as indicated. The strain analyzed was JE18955 (mddA⁺/pMDD8 mddA⁺). Growth curves were determined using a microplate reader (Bio-Tek Instruments) as described in Materials and Methods. The error bars represent standard deviations.