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. 2014 Dec 22;197(2):314–325. doi: 10.1128/JB.02311-14

TABLE 1.

Kinetic parameters of S. enterica MddAWTa

Substrate Km(app) (mM) kcat(app) (s−1) kcat/Km (M−1 s−1)
MSX 576 ± 89 35 ± 2 6 × 104
MSO 230 ± 56 31 ± 2 1 × 104
Acetyl-CoA 156 ± 41 23 ± 1 2 × 104
a

The apparent kinetic parameters of the SeMddAWT protein were determined using a spectrophotometric assay described in Materials and Methods. The catalytic variant SeMddAE82Q was used as a negative control.