TABLE 2.
Primer sequences
| Plasmid or strain constructed | Primer name | Sequence (5′ to 3′)a |
|---|---|---|
| pDPV447 | 593 | CTTCGGACCTACCTTTGAATTAGCACAAAAAAATAAAAAAGGTGG |
| 594 | CCACCTTTTTTATTTTTTTGTGCTAATTCAAAGGTAGGTCCGAAG | |
| 603 | GTCTTCTTTACATAAAAGCGAGCCTTTTACAAAACATAACC | |
| 604 | GCTTTACGTTCTTCCATAACTTTCACATCTGGATTG | |
| pDPV454 | 605 | GTTGAGATGGCACTCGAGTTAAATCGTGATCCTGCCG |
| 606 | CGGCAGGATCACGATTTAACTCGAGTGCCATCTCAAC | |
| 603, 604 | ||
| pDPV455 | 603, 604, 605, 606 | |
| pDPV458 | 612 | GTGGAGAACCTGTACTTCCAGGGTTCAAATGATACGGGCGC |
| 613 | CCACTTTGTACAAGAAAGTTGCATTTTAATACTTTTGAATGCCTAGTTTAAC | |
| pDPV459 | 614 | CGCGGATCCGATATTGAGGTCGAGTCATTTG |
| 615 | CGCCTGCAGCAATGACATGCGTATCATCAG | |
| pDPV460 | 616 | CGCAGATCTAAAGGACATATTTGTTCACCTATC |
| 617 | CGCAGATCTAAGTATTAAAAACAGGAGGGCCTAC | |
| DPVB668 | 607 | ACAAGTACGCAGGTGCTGGC |
| 608 | CGATTATGTCTTTTGCGCAGTCGGCCCTCACGTTCGTAATCCACC | |
| 609 | GAGGGTTGCCAGAGTTAAAGGATCCCTCCGCAGACCAATTAGGC | |
| 610 | GATACACCGATTGACGTACG |
a
Restriction sites are underlined, TEV cleavage site regions of pDEST-HisMBP-T are italicized and in boldface, and attR2.1 regions of pDEST-HisMBP-T are italicized and underlined.