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Single molecule binding event resolved as a wavelength shift in single nanoparticle spectroscopy. (a) Single AuNRs were illuminated by white light and the scattered spectrum was analyzed by a dark-field microscopy coupled with a spectrometer. (b) The binding of a single protein molecule onto a AuNR was resolved as the wavelength shift in the scattered spectrum. Reprinted with permission from Ref. 22. Copyright (2012) American Chemical Society.
