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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Anal Chem. 2013 Dec 12;86(1):2–14. doi: 10.1021/ac403890n

Fig. 6.

Fig. 6

Super-localization of single fluorescent molecules reveals the reactivity of single nanoparticles. (a) The fluorogenic reaction converts a non-fluorescent molecule (reactant) to a fluorescent molecule (product). (b) Such single molecule reaction results in the appearance of a single molecule fluorescence pattern and a burst in the fluorescence emission at certain location. (c) By counting the number of single molecule reaction events across the Au nanorod, one can map the sub-particle distribution of reactivity. (d) Substrate concentration-dependent turn-over rate in six sub-particle regions as defined in (c) exhibited quite different reaction kinetics. Adapted by permission from Macmillan Publishers Ltd:[Nature Nanotechnology] (Ref. 104), copyright (2012).