A, ChIP-reChIP with TRIM28 and RRP1B antibodies in MDA-MB-231 cells.
*; P ≤ 0.05 compared to both IgG/TRIM28 and IgG/RRP1B controls.
B, ChIP-reChIP with HP1α and RRP1B antibodies in MDA-MB-231 cells.
*; P ≤ 0.05 compared to both IgG/HP1α and IgG/RRP1B controls.
C, H3K9me3 ChIP-qPCR in MDA-MB-231 clonal isolates over-expressing RRP1B
and control cell lines. D, H3K9me3 ChIP-qPCR in MDA-MB-231 cells transfected
with either RRP1B siRNA or negative control siRNA. E, Venn diagram of unique
and common H3K9me3-enriched regions identified by ChIP-seq in MDA-MB-231 clonal isolates
over-expressing RRP1B and control cell lines.