Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Gene. 2014 Nov 8;556(1):74–79. doi: 10.1016/j.gene.2014.11.009

Fig. 4. Overexpression of BRCA1 represses the induction of Pol III genes by alcohol.

Fig. 4

MCF-7 cells were transfected with vector or wt BRCA1 expression construct as described in Fig. 1. The resultant cell lysates and total RNA from these cells was extracted. Immunoblot analysis was carried out to determine protein level of BRCA1 (A). RT-qPCR was performed to measure the levels of pre-tRNALeu (B), 5S rRNA (C) transcription. The fold change was calculated by normalizing to the amount of GAPDH. The bars represent Mean ± SE of at least three independent determinations of transfection with the constructs. The results indicate that overexpression of BRCA1 decreases the induction of Pol III gene transcription. **: p<0.01.