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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Gene. 2014 Nov 8;556(1):74–79. doi: 10.1016/j.gene.2014.11.009

Fig. 5. Reduction of BRCA1 increases Pol III gene transcription.

Fig. 5

MCF-7 cells were transfected with mismatch RNA (mm RNA, as a control) or BRCA1 siRNA for 48 h. The resultant cell lysates and total RNAs from these cells was extracted. Immunoblot analysis was carried out to determine protein level of BRCA1 (A). RT-qPCR was performed to measure the levels of BRCA1 mRNA (B), pre-tRNALeu (C), 5S rRNA (D) transcription. The fold change was calculated by normalizing to the amount of GAPDH. The bars represent Mean ± SE of at least three independent determinations of transfection with mm RNA or BRCA1 siRNA. The results indicate that repression of BRCA1 by its siRNA increases the induction of Pol III gene transcription, compared to mm RNA. **: p<0.01.