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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Gene. 2014 Sep 26;556(1):13–18. doi: 10.1016/j.gene.2014.09.049

Figure 3.

Figure 3

Mod5-GFP aggregates formed under fluconazole selection do not affect nuclear tgm-silencing. (A) Cellular localization of Mod5-GFP (green) in untreated yeast contrasting with cytoplasmic aggregation of Mod5-GFP in yeast following 7 days of fluconazole-selection. (nuclear DAPI staining in blue) (B) Fluconazole-selection does not alleviate nuclear tRNA gene-mediated silencing relative to unselected controls (untreated). Yeast were transformed with a tgm-silencing reporter plasmid consisting of a URA3 selection marker and a tRNATyr (SUP4) gene which silences a nat1 resistance gene under the control of a Gal4 UAS and Gal1 basal promoter(Hull et al., 1994; Pratt-Hyatt et al., 2013).