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. Author manuscript; available in PMC: 2015 Dec 18.
Published in final edited form as: Mol Cell. 2014 Nov 26;56(6):777–785. doi: 10.1016/j.molcel.2014.10.020

Figure 3. The processing of R-loops by the endonucleases XPF and XPG is a general and conserved mechanism.

Figure 3

(A, B). P-KAP1 level in HeLa cells transfected with siXPG or siLUC 24 hours before transfection with siASF or siSETX. (C) γH2AX intensity in HeLa-TetON-RNase H1 cells transfected with siLUC or siXPG for 48 hours and treated for 2 hours with 5μM camptothecin. Doxycycline (500 ng/μl) was added in combination with siRNAs where indicated. a.u. = arbitrary units. (D) Frequencies of recombination in the LY direct-repeat system. Each data represents the median of 3-4 independent experiments. Error bars represent the standard error of the median (SEM, n=3-4). See also Figure S2 and Table S1.