Skip to main content
. Author manuscript; available in PMC: 2015 Dec 18.
Published in final edited form as: Mol Cell. 2014 Nov 26;56(6):777–785. doi: 10.1016/j.molcel.2014.10.020

Figure 4. R-loop processing requires TC-NER factors but not XPC.

Figure 4

(A) P-KAP1 level in HeLa cells transfected with siXPA or siLUC 24 hours before transfection with siLUC or siAQR. (B) P-KAP1 level in HeLa cells transfected with siXPB, siXPD or siLUC 24 hours before transfection with siLUC or siAQR. (C) P-KAP1 level in HeLa cells transfected with siXPC or siLUC 24 hours before transfection with siLUC or siAQR. (D) P-KAP1 level in HeLa cells transfected with siCSB or siLUC 24 hours before transfection with siLUC or siAQR. (E) Model for how an R-loop is processed into a DSB. The stalling of the RNA polymerase allows CSB to recruit the endonucleases XPF and XPG. XPF and XPG generate a gap that can be converted into a DSB through DNA replication and/or XPF and XPG cleave the R-loop on both strands producing a DSB. See also Figure S3.