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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Sep 26;453(3):309–315. doi: 10.1016/j.bbrc.2014.09.053

Table 1.

TOPO sequencing revealed the enriched dengue thioaptamer sequences. The 24-base random region is in the middle. The thiol-modification is at the 5′ end of adenine (A) present in the random region and the 3′ end primer region. The 5′ end primer region does not have the thiol-modification since it originated from the primers used in the PCR reaction which were not thiol-modified.

Name Sequence (5′ - 3′)
DENTA-1 CGGCATTCTCCTGCTACGAGG-CGCTGCGGTACACCCCGACTCCAC – GAGCCACTGTCTACGGACATCTG
DENTA-2 CGGCATTCTCCTGCTACGAGG-CCTCCCACATATAGTCCCCCTCTA – GAGCCACTGTCTACGGACATCTG
DENTA-3 CGGCATTCTCCTGCTACGAGG-GGACCCGTTACCGCGACAACCGCA – GAGCCACTGTCTACGGACATCTG
DENTA-4 CGGCATTCTCCTGCTACGAGG-CCACAACACACACCTGCTGCAAAC-GAGCCACTGTCTACGGACATCTG