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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Transplantation. 2015 Jan 15;99(1):48–55. doi: 10.1097/TP.0000000000000476

Figure 5.

Figure 5

RPM protects hepatocytes via the mTORC2-Akt pathway, (a) mTOR inhibitors regulate hepatocyte mTORC2-Akt signaling pathway. Hepa1 cells were incubated with vehicle (Ctl) or RPM, or Torin 1 or Tg, or RPM/Tg, or Torin 1/Tg, as described in the material and methods. Cell lysates were collected at 6h, and analyzed by Western blots to measure levels of phosphorylated S6K, Akt (S473) and LC3B. 2 replicates/expt. group, (b) Akt inhibition abrogated cytoprotective effect of RPM. Hepa1 cells were incubated with vehicle (Ctl) or Tg, or RPM, or RPM/Tg, or TCN or RPM/TCN/Tg, as described in the material and methods. Cell death was measured at 24h by LDH assay. 3 replicates/expt. group.

Representative results of 2 different experiments. *p<0.05, **p<0.01