TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype(s) and/or description | Reference |
|---|---|---|
| Strains | ||
| V. parahaemolyticus | ||
| RIMD 2210633 | WT O3:K6 clinical isolate; Smr | 29 |
| SOY1456 (ΔVP1456) | WT with a VP1456 deletion | This study |
| SOY1723 (ΔVP1723) | WT with a VP1723 deletion | This study |
| SOY1905 (ΔVP1905) | WT with a VP1905 deletion | This study |
| SOYA0356 (ΔVPA0356) | WT with a VPA0356 deletion | This study |
| SOYBCCT12 | WT with ΔVP1456 ΔVP1723 | This study |
| SOYBCCT124 | WT with ΔVP1456 ΔVP1723 ΔVPA0356 | This study |
| SOYBCCT123 | WT with ΔVP1456 ΔVP1723 ΔVP1905 | This study |
| SOYBCCT13 | WT with ΔVP1456 ΔVP1905 | This study |
| SOYBCCT34 | WT with ΔVP1905 ΔVPA0356 | This study |
| SOYBCCT134 | WT with ΔVP1456 ΔVP1905 ΔVPA0356 | This study |
| SOYBCCT234 | WT with ΔVP1723 ΔVP1905 ΔVPA0356 | This study |
| SOYBCCT1342 | WT with ΔVP1456 ΔVP1723 ΔVP1905 ΔVPA0356 | This study |
| Escherichia coli | ||
| DH5α-λpir | λpir ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1 | |
| DH5α-λpir(pJETΔVP1456) | pJET-ΔVP1456 transformed into E. coli DH5α-λpir | This study |
| DH5α-λpir(pJETΔVP1723) | pJET-ΔVP1723 transformed into E. coli DH5α-λpir | This study |
| DH5α-λpir(pJETΔVP1905) | pJET-ΔVP1905 transformed into E. coli DH5α-λpir | This study |
| DH5α-λpir(pJETΔVPA0356) | pJET-ΔVPA0356 transformed into E. coli DH5α-λpir | This study |
| β2155 DAP auxotroph | Donor for bacterial conjugation; thr1004 pro thi strA hsdS lacZΔM15 (F′ lacZΔM15 lacTRQJΔ36 proA+ proB+) ΔdapA Ermr pirRP4 (Kmr from SM10) | |
| β2155(pDSΔVP1456) | pDSΔVP1456 transformed into E. coli β2155 | This study |
| β2155(pDSΔVP1723) | pDSΔVP1723 transformed into E. coli β2155 | This study |
| β2155(pDSΔVP1905) | pDSΔVP1905 transformed into E. coli β2155 | This study |
| β2155(pDSΔVPA0356) | pDSΔVPA0356 transformed into E. coli β2155 | This study |
| MKH13 | MC4100 (ΔbetTIBA) Δ(putPA)101 Δ(proP)2 Δ(proU); Spr | 31 |
| MKH13(pBBVP1456) | pBBVP1456 transformed into MKH13 | This study |
| MKH13(pBAVP1723) | pBAVP1723 transformed into MKH13 | This study |
| MKH13(pBAVP1905) | pBAVP1905 transformed into MKH13 | This study |
| MKH13(pBAVPA0356) | pBAVPA0356 transformed into MKH13 | This study |
| Plasmids | ||
| pDS132 | R6K γori mobRP4 sacB Cmr; suicide vector | 38 |
| pDSΔVP1456 | ΔVP1456 cloned into pDS132 suicide vector | This study |
| pDSΔVP1723 | ΔVP1723 cloned into pDS132 suicide vector | This study |
| pDSΔV1905 | ΔV1905 cloned into pDS132 suicide vector | This study |
| pDSΔVPA0356 | ΔVPA0356 cloned into pDS132 suicide vector | This study |
| pJET1.2 | General cloning vector (Fermentas); Ampr | |
| pJETΔVP1456 | ΔVP1456 cloned into pJET1.2 vector | This study |
| pJETΔVP1723 | ΔVP1723 cloned into pJET1.2 vector | This study |
| pJETΔVP1905 | ΔVP1905 cloned into pJET1.2 vector | This study |
| pJETΔVPA0356 | ΔVPA0356 cloned into pJET1.2 vector | This study |
| pBBR1MCS | Broad-host-range cloning vector; Cmr | 39 |
| pBBVP1456 | VP1456 cloned into pBBR1MCS | This study |
| pBAD33 | Expression vector; AraC Cmr | |
| pBAVP1723 | VP1723 cloned into pBAD33 | This study |
| pBAVP1905 | VP1905 cloned into pBAD33 | This study |
| pBAVPA0356 | VPA0356 cloned into pBAD33 | This study |