TABLE 2.
Primers used in this studya
| Target | Primer | Sequence (5′→3′) |
|---|---|---|
| Cloning | ||
| VP1456 (BCCT1) | VP1456F (XbaI) | TCTAGATCTTGTGAGTTGAAGACACTTG |
| VP1456R (HindIII) | AAGCTTAAAAATGCCGAGCAATGAAT | |
| VP1723 (BCCT2) | VP1723F (XbaI) | TCTAGAAACTTGTGCTTGGTGATGTG |
| VP1723R (SacI) | GAGCTCACGGCACACTTTCGCATG | |
| VP1905 (BCCT3) | VP1905F (KpnI) | GAGAGGTACCGATCTTCCGCTTTCAC |
| VP1905R (PstI) | GAGACTGCAGAGCAGGGTGCTGGCTTC | |
| VPA0356 (BCCT4) | VPA0356F (XbaI) | TCTAGAAGCGGCTTTTTGAACATCCT |
| VPA0356R (HindIII) | AAGCTTCCAATTAAGGGCTCTTTGCAT | |
| SOE PCR | ||
| VP1456 | VP1456A (XbaI) | TCTAGAATCAATGGGGACAGCGATAA |
| VP1456B | cagctgagatctggtaccCGAAGCGAATTTTATCACCAA | |
| VP1456C | ggtaccagatctcagctgCGTACCGAACTTTCCGCTTA | |
| VP1456D (SacI) | GAGCTCCAACCATTTTCGCGTTTGTTC | |
| VP1456FL-F | GTCGATTACAATGGCGGATT | |
| VP1456FL-R | GTGGCACATTGTGAATGCTC | |
| VP1723 | VP1723A (XbaI) | TCTAGAACGATATGGTCTGCCAGCTT |
| VP1723B | cagctgagatctggtaccGGGACGTTTAATCCCACCAT | |
| VP1723C | ggtaccagatctcagctgGGTCTAATGGATGAACCTCGTC | |
| VP1723D (SacI) | GAGCTCCCAATTTCTGGATAAAGCACCC | |
| VP1273FL-F | TGCGCTTTTAAACACCATTG | |
| VP11723FL-R | ATGTCCAACGGAGGACAATC | |
| VP1905 | VP1905A (XbaI) | TCTAGAGAGGAACGATGACAAAGGGTA |
| VP1905B | cagctgagatctggtaccCGAGCCAAGACATGAATGAA | |
| VP1905C | ggtaccagatctcagctgATGTTTGATGTGCTGCCATTT | |
| VP1905D (SacI) | GAGCTCTTGATCGATTATTGACGCTCTG | |
| VP1905FL-F | AATAGCGCGGATGATCTGAC | |
| VP1905FL-R | TTGAATGCGCTTGCAATATC | |
| VPA0356 | VPA0356A (XbaI) | TCTAGACTTGATGTGAGGGGAAATGC |
| VPA0356B | cagctgagatctggtaccTGTGTCGATGTCTGGTTTCG | |
| VP0356C | ggtaccagatctcagctgATCATTTCGGTGCTGTTCTTG | |
| VP0356D (SacI) | GAGCTCTTTGCATTTTATGGGGTTGG | |
| VPA0356FL-F | GCCCACTTCAAACTGTCGTT | |
| VPA0356FL-R | CTCGATTCGATGTCATTCCA | |
| VP1456 | VP1456RT-F | GTTCGGTCTTGCGACTTCTC |
| VP1456RT-R | CCCATCGCAGTATCAAAGGT | |
| VP1723 | VP1723RT-F | AACAAAGGGTTGCCACTGAC |
| VP1723RT-R | TTCAAACCTGTTGCTGCTTG | |
| VP1905 | VP1905RT-F | TGGACGGTATTCTACTGGGC |
| VP1905RT-R | CGCCTAACTCGCCTACTTTG | |
| VPA0356 | VPA0356RT-F | CAAGGCGTAGGCCGCATGGT |
| VPA0356RT-R | ACCGCCCACGATGCTGAACC | |
| VPr001 (16S rRNA) | VPr001RT-F | ACCGCCTGGGGAGTACGGTC |
| VPr001RT-R | TTGCGCTCGTTGCGGGACTT |
a
Underlining indicates restriction enzyme sites; nucleotides in lowercase type indicate complementary overlaps. Abbreviations: F, forward; R, reverse; RT, reverse transcriptase; FL, flanking.