FIG 9.
Δfur mutants accumulate intracellular iron. (A) Growth of streptonigrin-treated cells. (B) Growth of untreated cells. Strains were grown overnight in 2 ml of LB medium, diluted 1:20 in fresh LB medium, and divided into untreated and treated samples. Streptonigrin was added to the treated samples at a final concentration of 1 μM. Cell growth was measured as the OD600 over a period of 24 h, while incubating at 28°C with shaking at 180 rpm. Error bars represent the standard deviations between replicates of each strain.