TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| Pantoea stewartii subsp. stewartii | ||
| DC283 | Wild type; Nalr | 58 |
| ΔiucA mutant | Deletion in iucA; Nalr Kanr | This study |
| ΔiutA mutant | Deletion in iutA; Nalr Kanr | This study |
| Δfur mutant | Deletion in fur; Nalr | This study |
| ΔiucA/iucA+ mutant | Deletion in iucA complemented with pBBR1-MCS4GW:iucA (pLPB44) | This study |
| ΔiutA/iutA+ mutant | Deletion in iutA complemented with pBBR1-MCS4GW:iutA (pLPB43) | This study |
| Δfur/fur+ mutant | Cmr Nalr; deletion in fur complemented by chromosomal integration of wild-type fur into intergenic region downstream of glmS | This study |
| DC283(pHC60) | Wild-type DC283 constitutively expressing GFP | This study |
| ΔiucA mutant(pHC60) | ΔiucA strain constitutively expressing GFP | This study |
| ΔiutA mutant(pHC60) | ΔiutA strain constitutively expressing GFP | This study |
| ΔiucA/iucA+ mutant(pHC60) | ΔiucA/iucA+ strain constitutively expressing GFP | This study |
| ΔiutA/iutA+ mutant(pHC60) | ΔiutA/iutA+ strain constitutively expressing GFP | This study |
| Escherichia coli | ||
| DH10β | F− endA1 recA1 galE15 galK16 nupG rpsL lacX74 80dlacZΔM15 araD139(ara-leu)7697 mcrA(mrr-hsdRMS-mcrBC) | Invitrogen |
| S17-1pir+ | RP4, Mob+; Smr | 59 |
| EC100pir+ | F− mcrA(mrr-hsdRMS-mcrBC) 80dlacZΔM15 lacX74 recA1 endA1 araD139(ara-leu)7697 galU galK-rpsL nupG pir+(DHFR) | Epicentre |
| Plasmids | ||
| pUC18R6K-mini-Tn7-cat | Cmr Apr | 36 |
| pAUC40 | Cmr Smr | 34 |
| pFLP2 | sacB+; FLP recombinase; Apr | 60 |
| pCR8/GW/TOPO | Cloning vector; Spr | Invitrogen |
| pKD4 | Kanr | 35 |
| pBBR1-MCS4GW | Gateway compatible broad-host-range cloning vector | 34 |
| pLPB02 | fur::kan cloned into pCR8/GW/TOPO; Kanr Spr | This study |
| pLPB04 | fur::kan cloned into pAUC40; Kan | This study |
| pLPB29 | fur ORF+promoter cloned into pUC18R6K-mini-Tn7-cat; Cmr Apr | This study |
| pLPB34 | iucA::cloned into pCR8/GW/TOPO | This study |
| pLPB35 | iutA::kan cloned into pCR8/GW/TOPO | This study |
| pLPB36 | iucA::kan cloned into pAUC40 | This study |
| pLPB37 | iutA::kan cloned into pAUC40 | This study |
| pLPB41 | iutA ORF cloned into pCR8/GW/TOPO | This study |
| pLPB42 | iucA ORF+promoter cloned into pCR8/GW/TOPO | This study |
| pLPB43 | iutA ORF cloned into pBBR1-MCS4GW | This study |
| pLPB44 | iucA ORF+promoter cloned into pBBR1-MCS4GW | This study |
| pHC60 | Tetr; constitutively expresses GFP | 38 |
a
Cmr, chloramphenicol resistance; Tetr, tetracycline resistance; Nalr, nalidixic acid resistance; Apr, ampicillin resistance; Spr, spectinomycin resistance; Smr, streptomycin resistance; Kanr, kanamycin resistance.