TABLE 1.
G. kaustophilus strains
| Strain | Relevant descriptiona | Doubling time (min)b |
|---|---|---|
| MK242 | Derivative of the wild-type strain HTA426; ΔpyrF ΔpyrR ΔhsdM1S1R1 Δ(mcrB1-mcrB2-hsdM2S2R2-mrr) GK0707::Pgk704-bgaB | ND |
| MK480 | Derivative of the strain MK242; ΔpyrF ΔpyrR ΔhsdM1S1R1 Δ(mcrB1-mcrB2-hsdM2S2R2-mrr) GK0707::Pgk704-bgaB Δ(mutS-mutL) ΔmutY Δung Δmfd | ND |
| MK242p70 | MK242 derivative; trpE::pGKE70 | 20.2 ± 0.5 |
| MK480p70 | MK480 derivative; trpE::pGKE70 | 21.8 ± 0.5 |
| ΔmutSLp70 | MK242 derivative; Δ(mutS-mutL) trpE::pGKE70 | 20.4 ± 0.8 |
| ΔmutMp70 | MK242 derivative; ΔmutM trpE::pGKE70 | 21.6 ± 0.8 |
| ΔmutYp70 | MK242 derivative; ΔmutY trpE::pGKE70 | 20.0 ± 1.0 |
| ΔmutTp70 | MK242 derivative; ΔmutT trpE::pGKE70 | 21.9 ± 1.8 |
| Δungp70 | MK242 derivative; Δung trpE::pGKE70 | 20.4 ± 0.7 |
| Δmfdp70 | MK242 derivative; Δmfd trpE::pGKE70 | 22.7 ± 1.6 |
| ΔmutSLcm | MK242 derivative; Δ(mutS-mutL) trpE::pGKE70-mutSL | 21.9 ± 0.3 |
| ΔmutMcm | MK242 derivative; ΔmutM trpE::pGKE70-mutM | 22.6 ± 1.5 |
| ΔmutYcm | MK242 derivative; ΔmutY trpE::pGKE70-mutY | 22.9 ± 1.0 |
| ΔmutTcm | MK242 derivative; ΔmutT trpE::pGKE70-mutT | 22.9 ± 0.7 |
| Δungcm | MK242 derivative; Δung trpE::pGKE70-ung | 20.5 ± 0.9 |
| Δmfdcm | MK242 derivative; Δmfd trpE::pGKE70-mfd | 20.7 ± 0.7 |
| MK242BSpyrF | MK242 derivative; trpE::pGKE70-BSpyrFwt | ND |
| MK480BSpyrF | MK480 derivative; trpE::pGKE70-BSpyrFwt | ND |
Δ(mutS-mutL) encodes codons mutS1–106 fused with mutL534–630. ΔmutM, ΔmutY, ΔmutT, Δung, and Δmfd are deleted in codons mutM11–255, mutY14–347, mutT13–150, ung16–218, and mfd33–1060, respectively. trpE is an internal gene for anthranilate synthase component I. The strains MK242 and MK480 lack the native pyrF gene encoding orotidine-5′-phosphate decarboxylase, but MK242BSpyrF and MK480BSpyrF harbor BSpyrFwt, which encodes orotidine-5′-phosphate decarboxylase in B. subtilis.
G. kaustophilus strains were cultured at 60°C in LB medium with monitoring of OD600. The binary logarithm of OD600 data during the logarithmic growth phase was plotted against incubation time to calculate doubling times. The data are presented as means ± standard deviations (SD) (n = 3 to 16). ND, not determined.