TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′) | Target region | Underlined site |
|---|---|---|---|
| 0048F | GCCGCATGCTTTCGTTGCATCGCTATTTAG | mfd full sequence | SphI |
| 0048R | GCCGGATCCTTATGCCGTCACCGACTTCTC | mfd full sequence | BamHI |
| 0048upF | GCCGGATCCCTTCGTGAAGGCGGGCGCAC | mfd upstream | BamHI |
| 0048upR | GACCATTTCCTCACGCAGCCCGGCGACAAGCTG | mfd upstream | |
| 0048dwF | CAGCTTGTCGCCGGGCTGCGTGAGGAAATGGTC | mfd downstream | |
| 0048dwR | GGCGGATCCCGACGATGCCATAAATCGGG | mfd downstream | BamHI |
| 0463F | GCCCCTGCAGGCACAAGAGAAACAGAGCG | mutY full sequence | Sse8387I |
| 0463R | GCCGGATCCTTAATCGGGGCGGCGTAC | mutY full sequence | BamHI |
| 0463upF | GCCGGATCCCTTGGATTTGGCGGATGAAC | mutY upstream | BamHI |
| 0463upR | CCAAACACGTTGATGAAACTCGCGCGCTGG | mutY upstream | |
| 0463dwF | CCAGCGCGCGAGTTTCATCAACGTGTTTGG | mutY downstream | |
| 0463dwR | GCCGGATCCACATTCGTCCATGCGTGCTC | mutY downstream | BamHI |
| 1306F | GGTGTGTACGATGGCATAATCCCCCTCCTGTC | mutSL full sequence | |
| 1306R | GACAGGAGGGGGATTATGCCATCGTACACACC | Pgk704 fragment | |
| 1306upF | GCCGGATCCTCCACCAAAACGAAAAAGTG | mutS upstream | BamHI |
| 1306upR | CCACGTCGGATGGGAGAGCTGCACAACTTC | mutS upstream | |
| 1307R | GCCAGATCTCTACATCACCCGTTTAAATAG | mutSL full sequence | BglII |
| 1307dwF | GAAGTTGTGCAGCTCTCCCATCCGACGTGG | mutS downstream | |
| 1307dwR | GCCGGATCCATACCGCGTTTTCCAATAGG | mutS downstream | BamHI |
| 2204F | GCCGAATTCTGACGAATCGCCGTGGGCGC | trpE internal sequence | EcoRI |
| 2204R | GGCGAATTCCTGTCCGGATGGCGATGCAC | trpE internal sequence | EcoRI |
| 2728F | GCCGCATGCCGGAATTGCCGGAGGTG | mutM full sequence | SphI |
| 2728R | GCCGGATCCCTAGCGCTGGCAGCGCGGGC | mutM full sequence | BamHI |
| 2728upF | GCCGGATCCGCCGGAGTGAAAGTGGATAC | mutM upstream | BamHI |
| 2728upR | GACCGTTTTTTCAATGATCGTTTCCACCTC | mutM upstream | |
| 2728dwF | GAGGTGGAAACGATCATTGAAAAAACGGTC | mutM downstream | |
| 2728dwR | GCCGGATCCTAATCGCAGAATGCGGATTC | mutM downstream | BamHI |
| 3067F | GCCCCTGCAGGCGTGAACGAATTGCAACGGG | mutT full sequence | Sse8387I |
| 3067R | GCCGGATCCTCAGCTCGGATCGAGCCG | mutT full sequence | BamHI |
| 3067upF | GCCGGATCCAATACATGTGGCGCCGACAG | mutT upstream | BamHI |
| 3067upR | GAGCCGATATGAAAGGTTTGTCACCCGTTG | mutT upstream | |
| 3067dwF | CAACGGGTGACAAACCTTTCATATCGGCTC | mutT downstream | |
| 3067dwR | GCCGGATCCTTCGCCTTGCATACTTCCTG | mutT downstream | BamHI |
| 3421F | GCCGCATGCCGATTCTCAAAAACGAC | ung full sequence | SphI |
| 3421R | GCCGGATCCTCATTCAGCGCGGGCGCCGATG | ung full sequence | BamHI |
| 3421upF | GCCGGATCCCTTGGATGTACCATGCTTTG | ung upstream | BamHI |
| 3421upR | GTTCTCGATTTGCCACTCCTCTTCAAGCAG | ung upstream | |
| 3421dwF | CTGCTTGAAGAGGAGTGGCAAATCGAGAAC | ung downstream | |
| 3421dwR | GCCGGATCCTTTCATCCGATGCGACGTCC | ung downstream | BamHI |
| P704F | GCCAAGCTTTTTCTTTTTCCTCCTTTGTTATC | Pgk704 fragment | HindIII |
| pyrFF | GTACATATGCGAAACAACCTGCCCATC | BSpyrFwt sequence | NdeI |
| pyrFR | GCCGGATCCTTAAGATTTGATTCCCTCCC | BSpyrFwt sequence | BamHI |