TABLE 3.
Mutations generated in G. kaustophilus mutants
| Mutation | Mutation frequencya |
|||||
|---|---|---|---|---|---|---|
| MK242p70 | ΔmutSLp70 | ΔmutYp70 | Δungp70 | Δmfdp70 | MK480p70 | |
| rpoB mutations in Rifr | ||||||
| T1393C | 3/48 | 2/19 | ||||
| C1405A | 2/48 | 1/22 | 3/21 | |||
| A1406G | 5/20 | |||||
| A1406T | 3/48 | |||||
| G1414T | 2/48 | |||||
| A1415T | 2/48 | |||||
| A1416T | 1/48 | |||||
| C1433A | 3/22 | |||||
| C1433T | 2/48 | 1/19 | 1/20 | |||
| C1444G | 1/21 | |||||
| C1444T | 14/48 | 2/19 | 3/22 | 3/21 | 1/20 | |
| A1445G | 4/48 | 11/19 | 1/21 | |||
| A1445T | 1/48 | |||||
| C1460A | 1/48 | 1/19 | 15/22 | 24/32 | ||
| C1460T | 13/48 | 2/19 | 13/21 | 13/20 | 8/32 | |
| rpsL mutations in Strr | ||||||
| A167C | 2/48 | 9/21 | ||||
| A167G | 18/48 | 1/22 | 2/22 | 10/21 | ||
| A168T | 1/48 | |||||
| A301G | 13/48 | 4/22 | 6/22 | 7/22 | 1/21 | 20/32 |
| A302G | 11/48 | 10/22 | 4/22 | 10/22 | 1/21 | 3/32 |
| C311A | 3/48 | 1/22 | 9/22 | 1/22 | ||
| C311T | 2/22 | 1/22 | 7/32 | |||
| Not identified | 6/22 | 1/22 | 1/22 | 2/32 | ||
| Mutation spectra | ||||||
| A · T→C · G | 2/96 (2.1) | 9/41 (22) | ||||
| A · T→G · C | 49/96 (51) | 28/35 (80) | 10/43 (23) | 20/42 (48) | 17/41 (41) | 23/62 (37) |
| A · T→T · A | 8/96 (8.3) | |||||
| C · G→A · T | 8/96 (8.3) | 2/35 (5.7) | 28/43 (65) | 4/42 (9.5) | 24/62 (39) | |
| C · G→G · C | 1/42 (2.4) | |||||
| C · G→T · A | 29/96 (30) | 5/35 (14) | 5/43 (12) | 17/42 (40) | 15/41 (37) | 15/62 (24) |
a
G. kaustophilus mutants that were grown on LB plates containing 50 mg liter−1 Rif or Str were analyzed for rpoB and rpsL sequences, respectively. The fraction represents sequences carrying the mutation per total number of sequences determined. Mutation spectra summarize sequences that arise from the mutation per total sequences carrying mutations (excluding those not identified). Values in parentheses indicate percentages (%). Empty cells indicate that the mutation was not observed.