TABLE 1.

Oligonucleotides used in this study

Name	Sequence (5′–3′)a	Amplimer size (bp)	Function or description	Reference or source
tRNAup	TCTGCATTATGCGTTGTT	224	Upstream stx2A	This study
Stx2a-gfpTir5	TAAAGTTAATCAGAATTCAAAACCCAGTAACAGGCAC		Overlapping of gfp gene and stx2A	This study
GfpTir up	GAATTCTGATTAACTTTA	753	Entire gfp gene from pGreen TIR vector	This study
GfpTir lp	TTATTTGTAGAGCTCATCC
GfpTir lp-cm5	GAAGCAGCTCCAGCCTACACATTATTTGTAGAGCTCATCC		Overlapping of gfp gene and cat
Gfp short-up	TCCATCTTCAATGTTGTGTCT	211	gfp fragment for qPCR standard	This study
Gfp short-lp	GAACTATAAGACACGTGCTGA
Cm 5	TGTGTAGGCTGGAGCTGCTTC	1015	cat gene	31
Cm-3	CATATGAATATCCTCCTTAG
Cm3-stx	CTAAGGAGGATATTCATATGAGGAGTTAAGTATGAAGAAG	281	Overlapping cat-stx	31
Stx2Blp	TCAGTCATTATTAAACTG		Final codon of stx2B	This study
UP378	GCGTTTTGACCATCTTCGT	378	378-bp stx2A fragment	32
LP378	ACAGGAGCAGTTTCAGACAG
RR46 LP	GAGCTCTAAGGAGGTTAT	457	Red recombinase in pKD46	31
RR46-UP	GTGCAGTACTCATTCGTT
GFPTir-F	GCTTCCATCTTCAATGTTGTGTCT	121	Real-time PCR for gfp gene	This study
GFPTir-R	CATTCTTGGACACAAATTGGAATACAACT
GFPTir- probe	FAM-CATGGCAGACAAACAA-NFQ
STX-Any f	ACGGACAGCAGTTATACCACTCT	65	Real-time PCR for stx2 gene	29
STX-Any r	ACGTTCCGGAATGCAAATCAG
STX-Any probe	FAM- CCAGCGCTGCGACACG-NFQ

^aOverlap region is underlined.