TABLE 2.
Key mutations
| Genea | Mutation | Appearance location | Replacing mutation (within same expt) | Appearance location | n | Expt(s) |
|---|---|---|---|---|---|---|
| pyrE/rph | Δ82bp | Jump 1, pre-jump 1 | 8 | 3, 5, 9, 10 4, 6, 7, 8 | ||
| rpoB | E672K (GAA→AAA) | Jump 1 | 8 | 3, 5, 9 | ||
| P1100Q (CCG→CAG) | Jump 1 | 4, 8 | ||||
| E546V (GAA→GTA) | Jump 1 | 10 | ||||
| H673Y (CAC→TAC) | Jump 1 | D785Y (GAC→TAC) | Jump 2 | 6 | ||
| L671P (CTG→CCG) | Jump 1 | Hypermutator | Jump 2 | 7 | ||
| hns/tdk | Intergenic (−114/–487) IS2 | Jump 2 | 7 | 3 | ||
| Intergenic (−110/–488) IS1 | Jump 2 | 4 | ||||
| Intergenic (−274/–328) IS5 | Jump 2 | 5 | ||||
| Intergenic (−86/–511) IS1 | Post-jump 2 | 6 | ||||
| Intergenic (−67/-531) IS1 | Jump 2 | 8 | ||||
| Intergenic (−93/–505) IS1 | Jump 3 | 9 | ||||
| Intergenic (−258/–344) IS5 | Jump 2 | Intergenic (−274/–328) IS5 | Post-Jump 2 | 10 | ||
| corA | Coding (726–728/951 nt) Δ3bp | Jump 1 | Coding (220–224/951 nt) Δ5bp | Jump 1 | 3 | 4 |
| A206V (GCG→GTG) | Jump 1/2 | Coding (113–211/951 nt) Δ99bp | Jump 2 | 5 | ||
| Coding (668/951 nt) duplication, 21 bp | Jump 2/3 | Wild type | Jump 3 | 10 | ||
| ygaZ | Coding (529–532/738 nt) IS5 | Jump 2 | Coding (307–316/738 nt) Δ10bp | Post-jump 3 | 3 | 3 |
| E49* (GAA→TAA) | Jump 3 | 9 | ||||
| 2807900, 19 bp × 2 | Post-jump 3 | |||||
| iap | Coding (98–101/1,038 nt) IS5 | Post-jump 2/3 | 6, 9 | |||
| metL | Coding (1,338/2,433 nt) Δ1bp | Jump 2/3 | A798E (GCG→GAG) | Jump 3 | 1 | 10 |
| ygeW | S200R (AGC→CGC) | Jump 1 | 2 | 5, 9 |
a
Genetic terms that incorporate a shill (/), such as “pyrE/rph”, refer to intergenic regions, e.g., “pyrE/rph” refers to the pyrE-rph intergenic region.