TABLE 4.
Key mutations in validation ALE
| Genetic regiona | Starting strain genotype | Mutation | No. of occurrences | Expt(s) |
|---|---|---|---|---|
| pyrE/rph | rpoB(E546V) | Δ82bp deletion | 1 | 2 |
| Δ1bp deletion | 1 | 3 | ||
| rpoB(E672K) | Δ82bp deletion | 3 | 4, 5, 6 | |
| rpoB | pyrE/rph | A679V (GCA→GTA) | 1 | 8 |
| V857E (GTG→GAG) | 1 | 9 | ||
| hns/tdk | pyrE/rph | Intergenic (−75/–522) IS1 | 1 | 9 |
| metL | rpoB(E546V) | W424* (TGG→TAG) | 1 | 1 |
a
Genetic expressions that incorporate a shill (/), such as “pyrE/rph”, refer to intergenic regions, e.g., “pyrE/rph” refers to the pyrE-rph intergenic region.