FIG 1.

Schematic illustration of experiments conducted to demonstrate the proximity effect between cellulases displayed on the surface of yeast cells. MIX-yeast is a mixture of 3 single-cellulase-displaying yeasts, EG-yeast (for example, blue), CBH-yeast (green), and BG-yeast (red), which are listed in Table 2. MIX-yeast was prepared by mixing the 3 single-cellulase-displaying yeasts in a ratio corresponding to the display ratio of ALL-yeast. The proximity effect between the cellulases displayed on ALL-yeast, which simultaneously displays EG, CBH, and BG, was determined by comparing the PASC-degrading activity of ALL-yeast and MIX-yeast.