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. 2014 Dec 11;81(1):59–66. doi: 10.1128/AEM.02864-14

FIG 3.

FIG 3

Confirmation of cellulase display by immunostaining and fluorescence microscopy. Cells of 5 yeast strains were immunostained using an anti-FLAG mouse monoclonal primary antibody and CF405S-conjugated goat antimouse monoclonal secondary antibodies. Fluorescence microscopy was used to examine the fluorescence of EGFP on CBH-yeast and ALL-yeast, CF405S on EG-yeast and ALL-yeast, and mCherry on BG-yeast and ALL-yeast. Bars = 5 μm.