TABLE 1.
Plasmids and primers used in this study
| Plasmid or primer | Feature or sequencea |
|---|---|
| Plasmids | |
| pRS425DF-EG | Cell surface display of EG II with FLAG tag, LEU2 |
| pRS426DE-CBH | Cell surface display of CBH II with EGFP, URA3 |
| pRS423Dm-BG | Cell surface display of BG I with mCherry, HIS3 |
| pRS402 | ADE2 |
| pRS403 | HIS3 |
| pRS405 | LEU2 |
| pRS406 | URA3 |
| pRS403DF-EG | Cell surface display of EG II with FLAG tag, HIS3 |
| pRS405DF-EG | Cell surface display of EG II with FLAG tag, LEU2 |
| pRS406DF-EG | Cell surface display of EG II with FLAG tag, URA3 |
| pRS403DE-CBH | Cell surface display of CBH II with EGFP, HIS3 |
| pRS405DE-CBH | Cell surface display of CBH II with EGFP, LEU2 |
| pRS406DE-CBH | Cell surface display of CBH II with EGFP, URA3 |
| pRS403Dm-BG | Cell surface display of BG I with mCherry, HIS3 |
| pRS405Dm-BG | Cell surface display of BG I with mCherry, LEU2 |
| pRS406Dm-BG | Cell surface display of BG I with mCherry, URA3 |
| Primers | |
| BglII-FLAG-linker-EG-F | 5′-ATGCAGATCTGATTACAAGGATGACGATGACAAGGGTGGATCTACTGTCTGGGGCCAGTGTG-3′ |
| EG-SphI-extra-R | 5′-TGCAGTCGGACGATGCGCATGCCTTTCTTGCGAGACACGAGCTG-3′ |
| BglII-CBH-F | 5′-ATGCAGATCTCAAGCTTGCTCAAGCGTCTGG-3′ |
| CBH-SphI-R | 5′-ATGCGCATGCCAGGAACGATGGGTTTGCGTTTG-3′ |
| SphI-EGFP-F | 5′-ATGCGCATGCGGTGGATCTGGTGGCGTGA-3′ |
| EGFP-SphI-R | 5′-ATGCGCATGCCTTGTACAGCTCGTCCATGCC-3′ |
| NotI-BG-F | 5′-ATGCGCGGCCGCGATGAACTGGCGTTCTCTCCTC-3′ |
| BG-SphI-R | 5′-ATGCGCATGCTTGCACCTTCGGGAGCGC-3′ |
| SphI-5linker-mCherry-F | 5′-ATGCGCATGCGGTGGATCTGGTGGCGTGAGCAAGGGCGAGGAG-3′ |
| mCherry-SphI-R | 5′-ATGCGCATGCCTTGTACAGCTCGTCCATGCC-3′ |
| infusion GAPDH promoter F | 5′-CGGGCCCCCCCTCGAGACCAGTTCTCACACGGAACAC-3′ |
| infusion GAPDH terminator R | 5′-ACCGCGGTGGCGGCCGCTTTGATTATGTTCTTTCTATTTGAATGAGATATGA-3′ |
a
Underlined sequences are restriction enzyme sites.