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. 2014 Dec 11;81(1):272–281. doi: 10.1128/AEM.02265-14

FIG 4.

FIG 4

Organization and transcription analysis of the vpp cluster. (A) RT-PCR analysis of regions I, II, III, and IV as shown in Fig. 3B. The genomic DNA of strain SJY1 (lane +) and double-distilled H2O (ddH2O) were used as positive and negative templates for PCR with oligonucleotide pairs for regions I, II, III, and IV. The RNA (lane RNA) and cDNA (lane cDNA) from Ochrobactrum sp. SJY1 cells grown in the presence of nicotine were used as the templates for PCR analysis. (B) Quantitative RT-PCR analysis of the genes in the vpp cluster. The relative expression levels of the vppB, vppH, and vppF genes were measured using RNA extracted from Ochrobactrum sp. SJY1 grown in the presence (black bars) or absence (gray bars) of nicotine. All data were normalized to the 16S rRNA and are expressed as fold change relative to the expression level in cells. Each value is the mean from three parallel replicates ± SD.