FIG 5.

Characterization of VppB. (A) Spectrum scanning and SDS-PAGE of purified VppB. (B) HPLC analysis of the reaction of VppB at 0 min and 20 min. (C) Temperature-dependent enzyme activity of VppB. (D) pH optimization of VppB. VppB was unstable and participated when the pH value was less than 6.5. (E) Kinetic studies of VppB, fitted to the Michaelis-Menten curve for 6HN. (F) Multiple alignment and phylogenetic analysis of VppB and related amine oxidases. The GenBank accession number for each protein is shown in parentheses. (D and E) The values are the means from three parallel replicates ± SD.