Figure 6.

Anti-tumor activity of BsAb-IR-28z engineered T-cells. A. BsAb-IR-28z + T lymphocytes produce inflammatory cytokines when redirected with antigen-bound frBsAbs. CD20 redirected BsAb-IR, but not GFP (control) T-cells produce high levels of IFNg against CD20-positive Ramos tumor cells. BsAb-IR-28z or GFP transduced T-cells were cultured overnight with an equal number of CD20frBsAb, or control frBsAbs pretargeted CD20-positive Ramos cancer cells. Overnight culture supernatants were analyzed for human IFNg cytokine by ELISA. Concentration of IFNg is expressed as mean ± SEM in pg/ml from triplicate wells. B. Cytokine bead-array analysis of cytokine production (pg/ml) by BsAb-IR-28z + T-cells or GFP+ T-cells. Supernatants from three independent cultures were harvested 16 h post-stimulation, pooled and assessed for measurable cytokine.